Applied and Environmental Microbiology (2004) 70, 4889-4898

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James A. Baum, Chi-Rei Chu, Mark Rupar, Gregory R. Brown, William P. Donovan, Joseph E. Huesing, Oliver Ilagan, Thomas M. Malvar, Michael Pleau, Matthew Walters and Ty Vaughn (2004)
Binary toxins from Bacillus thuringiensis active against the western corn rootworm, Diabrotica virgifera virgifera LeConte
Applied and Environmental Microbiology 70 (8), 4889-4898
Abstract: The western corn rootworm, Diabrotica virgifera virgifera LeConte, is a significant pest of corn in the United States. The development of transgenic corn hybrids resistant to rootworm feeding damage depends on the identification of genes encoding insecticidal proteins toxic to rootworm larvae. In this study, a bioassay screen was used to identify several isolates of the bacterium Bacillus thuringiensis active against rootworm. These bacterial isolates each produce distinct crystal proteins with approximate molecular masses of 13 to 15 kDa and 44 kDa. Insect bioassays demonstrated that both protein classes are required for insecticidal activity against this rootworm species. The genes encoding these proteins are organized in apparent operons and are associated with other genes encoding crystal proteins of unknown function. The antirootworm proteins produced by B. thuringiensis strains EG5899 and EG9444 closely resemble previously described crystal proteins of the Cry34A and Cry35A classes. The antirootworm proteins produced by strain EG4851, designated Cry34Ba1 and Cry35Ba1, represent a new binary toxin. Genes encoding these proteins could become an important component of a sustainable resistance management strategy against this insect pest.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): James A. Baum, Joseph E. Huesing

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Diabrotica virgifera
Bacillus thuringiensis Cry34B-toxin (entomopathogen) Diabrotica virgifera
Bacillus thuringiensis Cry35B-toxin (entomopathogen) Diabrotica virgifera
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