Environmental Entomology (1999) 28, 1177-1182

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Un Taek Lim and Joon-Ho Lee (1999)
Enzyme-linked immunosorbent assay used to analyze predation of Nilaparvata lugens (Homoptera: Delphacidae) by Pirata subpiraticus (Araneae: Lycosidae)
Environmental Entomology 28 (6), 1177-1182
Abstract: An enzyme-linked immunosorbent assay (ELISA) was developed to detect the consumption of Nilaparvata lugens (Stål) by the lycosid Pirata subpiraticus (Bösenberg et Strand). Antiserum was produced in an ICR mouse using an isolated antigen from the female N. lugens. In cross-reactivity tests against 5 other prey species and 7 spider species found in paddy fields, absorbances for most prey species and spiders were <25% of that caused by antigen from female N. lugens. Laodelphax striatellus (Fallen) (Homoptera: Delphacidae) showed ~36% absorbance. P. subpiraticus that fed on female N. lugens were distinguishable from those fed on other prey species by reference to an absorbance threshold derived from laboratory cross reactivity tests. The strength of the antibody reaction to N. lugens antigen was also measured for female P. subpiraticus spider that had consumed varying number of female N. lugens as prey. P. subpiraticus that consumed >1.4 female N. lugens gave a positive reaction. The maximum period for the detection of a positive ELISA reaction was 6 h at 27°C after female P. subpiraticus had fed on 3 female N. lugens. P. subpiraticus collected in fields where N. lugens occurred showed a higher frequency of positive reaction in the ELISA than those collected in fields where N. lugens did not occur, suggesting the practical value of this ELISA test.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Un Taek Lim, Joon-Ho Lee

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Nilaparvata lugens
Pirata subpiraticus (predator) Nilaparvata lugens
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