Journal of Plant Pathology (2017) 99, 361-369

K. Kalaiponmani, R. Thangavelu and G. Varun (2017)
Optimization of protein isolation and preliminary comparative proteomics of pathogenic Fusarium oxysporum f. sp. cubense (P-Foc) and non-pathogenic Fusarium oxysporum (np-Fo)
Journal of Plant Pathology 99 (2), 361-369
Abstract: Panama wilt of banana caused by F. oxysporum f. sp. cubense (Foc) is a devastating disease of banana and in severe condition causes 100% yield loss. In order to understand the molecular mechanism of pathogenicity for the effective management of the wilt disease, three protocols (MgCl2/CHAPS, TCA-Acetone and TCA/Phenol-SDS) were evaluated to extract protein from Foc mycelial tissue. Comparatively, TCA/Phenol-SDS method of protein precipitation and extraction resulted in highest protein quantity (0.76 ± 0.025 mg/g of mycelial tissue) and ca. 600 spots were identified in Coomassie Brilliant Blue (CBB) stained gels. A comparative proteomic study conducted on pathogenic F. oxysporum f. sp. cubense (P-Foc) (race 1, VCG 0124) and non-pathogenic Fusarium oxysporum (np-Fo) revealed a difference at the proteome level. In the comparative proteomics study, 23 spots were found to be differentially expressed with > 1.5 fold intensity differences between P-Foc and np-Fo. Preliminarily, six spots (five significantly upregulated and one unique protein) from P-Foc were randomly picked and subjected to MALDI-TOF MS analysis. Further, MASCOT search and functional annotation revealed that these upregulated proteins identified in P-Foc may be involved in invasive growth, signal transduction and pathogen persistence during adverse condition.
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Link to article at publishers website
Database assignments for author(s): Raman Thangavelu

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium oxysporum f.sp. cubense	Banana/plantain (Musa)	India