Arthropod-Plant Interactions (2016) 10, 311-322

Ana Sedlarevic, Filis Morina, Ivo Tosevski, Uros Gasic, Maja Natic, Jelena Jovic, Oliver Krstic and Sonja Veljovic-Jovanovic (2016)
Comparative analysis of phenolic profiles of ovipositional fluid of Rhinusa pilosa (Mecinini, Curculionidae) and its host plant Linaria vulgaris (Plantaginaceae)
Arthropod-Plant Interactions 10 (4), 311-322
Abstract: Rhinusa pilosa (Gyllenhal) is a highly specific weevil that induces stem galls on the common toadflax Linaria vulgaris Mill. females oviposit the eggs near the apex of a growing shoot. The act of oviposition is accompanied by secretion of an ovipositional fluid, which is considered to be cecidogen, directly involved in gall induction. The remains of cecidogenic fluid were collected from the surface of the oviposition point on the stem. We performed a comparative analysis of the phenolics extracted from cecidogen, the stem and galls of L. vulgaris and adult and larva of R. pilosa by HPLC-DAD. One compound with A _max at 273, 332 nm (R _t 30.65 min) was exclusively found in the methanol extract of cecidogen. To further characterize the cecidogen and stem phenolic profiles, we used UHPLC coupled with an OrbiTrap mass analyzer. Among 49 phenolic compounds extracted from both the ovipositional fluid and the plant, protocatechuic acid and two phenolic glycosides were exclusively found in cecidogen: diosmetin-O-acetylrutinoside and an unidentified compound. The unknown compound produced an MS² base peak at 387 and 327 and 267 m/z base peaks at MS³ and MS⁴ fragmentation, respectively, and had the molecular formula C₃₂H₃₁O₁₈. The plausible role of phenolic compounds in the induction of gall formation on L. vulgaris is discussed.
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Link to article at publishers website
Database assignments for author(s): Ivo Tosevski

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Linaria vulgaris (weed)
Rhinusa pilosa (weed bioagent)	Linaria vulgaris (weed)