Annals of Microbiology (2007) 57, 565-570

Zhenlun Li, Zhongkang Wang, Guoxiong Peng, Youping Yin, Hua Zhao, Yueqing Cao and Yuxian Xia (2007)
Regulation of extracellular acid phosphatase biosynthesis by culture conditions in entomopathogenic fungus Metarhizium anisopliae strain CQMa102
Annals of Microbiology 57 (4), 565-570
Abstract: Metarhizium anisopliae is an imperfect entomopathogenic fungus. Once invading into its host, M. anisopliae needs to absorb basic nutrients such as phosphorus from the host haemolymph. A large number of phosphorylated compounds in haemolymph cannot be directly utilised by the fungal cell and must be hydrolysed into available form by phosphatase before ingested. Aims of this paper were to investigate optimum fermentation conditions for production of acid phosphatase and phosphatase isoenzymes by Metarhizium anisopliae. The optimum fermentation conditions were: glucose, 20 g/l; (NH4)₂SO₄, 2 g/l; casein, 4 g/l; MgSO₄, 0.5 g; KCl, 0.5 g; microelement salt solution, 10 ml; inoculum size, 1 x 10⁷ spores per 100 ml medium; initial medium pH, 6.0. Under these conditions, the highest total acid phosphatase activity was 3.05 U/ml in 4 days at 27 °C and 160 rpm. Synthesis of the acid phosphatase was repressed by 0.01% inorganic phosphate in culture medium. The spectrum of isoenzymes produced by M. anisopliae varied depending on the phosphorus source employed in the culture. A specific isoform with pI 9.45 was induced by casein, and another isoform of pI 8.21 was induced by phytic acid and disodium phenyl phosphate.
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Link to article at publishers website
Database assignments for author(s): Yuxian Xia

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Metarhizium anisopliae (entomopathogen)