Pest Management Science (2016) 72, 1558-1565

Wei-Chao Ren, Hu Zhao, Wen-Yong Shao, Wei-Wei Ma, Jian-Xin Wang, Ming-Guo Zhou and Changjun Chen (2016)
Identification of a novel phenamacril-resistance-related gene by the cDNA-RAPD method in Fusarium asiaticum
Pest Management Science 72 (8), 1558-1565
Abstract:
BACKGROUND
Fusarium asiaticum, a dominant pathogen of Fusarium head blight (FHB) in East Asia, causes huge economic losses. Phenamacril, a novel cyanoacrylate fungicide, has been increasingly applied to control FHB in China, especially where resistance of F. asiaticum against carbendazim is severe. It is important to clarify the resistance-related mechanisms of F. asiaticum to phenamacril so as to avoid control failures, and to sustain the usefulness of the new product.
RESULTS
A novel phenamacril-resistance-related gene Famfs1 was obtained by employing the cDNA random amplified polymorphic DNA (cDNA-RAPD) technique, and was validated by genetic and biochemical assays. Compared with the corresponding progenitors, deletion of Famfs1 in phenamacril-sensitive or highly phenamacril-resistant strains caused a significant decrease in effective concentrations inhibiting radial growth by 50% (EC₅₀ value). Additionally, the biological fitness parameters (including mycelial growth under different stresses, conidiation, perithecium formation and virulence) of the deletion mutants attenuated significantly.
CONCLUSION
Famfs1 not only was involved in the resistance of F. asiaticum to phenamacril but also played an important role in adaptation of F. asiaticum to the environment. Moreover, our data suggest that the cDNA-RAPD method can be a candidate technique to clone resistance-related genes in fungi.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Chang-Jun Chen, Ming-Guo Zhou

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium asiaticum