Plant Disease (2002) 86, 1345-1349

From Pestinfo-Wiki
Revision as of 08:24, 24 April 2011 by Bernhard Zelazny (Talk | contribs) (command-line import)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to: navigation, search

C.I. Dovas, N.I. Katis and A.D. Avgelis (2002)
Multiplex detection of criniviruses associated with epidemics of a yellowing disease of tomato in Greece
Plant Disease 86 (12), 1345-1349
Abstract: Since 1997, a yellowing disease has been observed in greenhouse tomato (Lycopersicon esculentum). By 2001, the disease was widespread, including open field tomato crops, and in most cases its incidence was 80 to 90% or even 100%. Epidemics in glasshouses were mainly associated with high populations of the whitefly Trialeurodes vaporariorum and Bemisia tabaci, the major whitefly pests in vegetable crops in Greece. The main leaf symptoms were severe yellowing, rolling, and brittleness. Samples from symptomatic plants were analyzed by polymerase chain reaction (PCR) and shown to be infected with Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV) (family Closteroviridae, genus Crinivirus). TICV was found in 164 of 183 symptomatic samples, while ToCV was less representative (25/183). Sequence comparisons of the amplified 229-bp and 466-bp products revealed 99 and 100% identity with the reported sequences of TICV and ToCV, respectively. A reverse transcription (RT) multiplex PCR assay using a simple sample preparation procedure was developed to allow rapid, specific, and simultaneous detection of both ToCV and TICV sequences in two steps. The method involves a one-tube RT-PCR step in which the combination of primers amplifies part of the heat shock protein to homologue gene of both ToCV and TICV, followed by a multiplex nested PCR amplification. This is the first report of TICV and ToCV in Greece and, as far as we know, the first report of TICV in Europe.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Crisostomos I. Dovas

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.


Tomato infectious chlorosis virus Tomato (Solanum lycopersicum) Greece
Tomato chlorosis virus Tomato (Solanum lycopersicum) Greece
Retrieved from "https://wiki.pestinfo.org/w/index.php?title=Plant_Disease_(2002)_86,_1345-1349&oldid=37474"