Current Microbiology (2008) 57, 575-579

Stanley Gunawan, Danielle M. Tufts and Blake R. Bextine (2008)
Molecular identification of hemolymph-associated symbiotic bacteria in red imported fire ant larvae
Current Microbiology 57 (6), 575-579
Abstract: The red imported fire ant (RIFA) (Solenopsis invicta Buren), an exotic insect pest in Texas, has become well established throughout the eastern part of the state. More aggressive than native ant species, RIFA gradually have enlarged their range and spread north and west despite intense efforts to stop them. Symbiotic bacteria have an important relationship in the midgut of fourth instar RIFA larvae. However, the presence of symbiotic bacteria in hemolymph has not been explored. In this study, symbiotic bacteria isolated from the hemolymph of fourth instar larvae of RIFA were genetically identified in terms of genus using a partial sequence of the 16S rRNA gene. Using three different primer sets to amplify regions of the gyrA, gyrB, and SG850 genes, multiple species of the genus Bacillus were identified as inhabitants of fire ant hemolymph. Analysis of gyrA gene identified Bacillus cereus with a percentage match of 94.13-99.20% with DNA sequences from GenBank BLAST (http://www.ncbi.nih.gov/). Using the gyrB gene, Bacillus species were identified with a percentage match of 95.48% to 100% using DNA sequences from GenBank. Finally, analysis of the SG850 gene identified Bacillus cereus with a percentage match of 96.20% to 99.83% using DNA sequences from GenBank.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Danielle M. Tufts

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Solenopsis invicta		U.S.A. (mid S)