Difference between revisions of "Plant Disease (2010) 94, 1448-1452"
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{{Publication | {{Publication | ||
| − | |Publication authors=Kadriye Çaglayan, Çigdem Ulubas Serçe, Eminur Barutçu, Kamuran Kaya, Vicente Medina, Mona Gazel, Soner Soylu and Oguzhan Çaliskan | + | |Publication authors=[[Kadriye Caglayan|Kadriye Çaglayan]], [[Çigdem Ulubas Serçe]], Eminur Barutçu, Kamuran Kaya, [[Vicente Medina]], [[Mona Gazel]], [[Soner Soylu]] and Oguzhan Çaliskan |
| − | |Author Page=Kadriye Caglayan | + | |Author Page=Kadriye Caglayan, Çigdem Ulubas Serçe, Soner Soylu, Vicente Medina, Mona Gazel |
|Publication date=2010 | |Publication date=2010 | ||
| − | |dc:title=Comparison by sequence-based and electron microscopic analyses of [[ | + | |dc:title=Comparison by sequence-based and electron microscopic analyses of [[Emaravirus fici|fig mosaic virus]] isolates obtained from field and experimentally inoculated fig plants |
|Publication journal=Plant Disease | |Publication journal=Plant Disease | ||
|prism:volume=94 (12) | |prism:volume=94 (12) | ||
| Line 11: | Line 11: | ||
|Publication abstract=Fig mosaic disease (FMD) and the fig mite, ''Aceria ficus'', are widespread in different fig growing provinces of Turkey. Fig trees (''Ficus carica'') cv. Bursa siyahı (D1) and an unknown seedling (D2) that showed typical FMD symptoms and was heavily infested by fig mites were used as donor plants for attempted mite transmissions to healthy fig seedlings. Transmission electron microscopy observations of donor plant samples prior to the transmission tests were performed and showed the presence of double membrane bodies (DMBs) in the palisade mesophyll cells. Electron microscopy of all experimentally inoculated fig seedlings showed the same bodies. This result reinforced the suggestion that an agent that elicits the production of DMBs in infected cells is involved in the etiology of FMD. Double-stranded (ds)RNA analyses were also performed from experimentally inoculated plants, and dsRNAs with sizes approximately 1.30 and 1.96 kb were obtained. Reverse transcription-polymerase chain reaction (RT-PCR) products of 468 and 298 bp specific to Fig mosaic virus (FMV) were amplified from both donor and experimentally inoculated plants. BLAST analyses of nucleotide sequences of these fragments showed 90% identity with FMV for the donor plant and 94 to 96% for experimentally inoculated plants. According to these results, FMV is present in both donor and experimentally inoculated plants in Turkey, and this virus is transmissible by ''A. ficus'' from fig plant to fig plant. | |Publication abstract=Fig mosaic disease (FMD) and the fig mite, ''Aceria ficus'', are widespread in different fig growing provinces of Turkey. Fig trees (''Ficus carica'') cv. Bursa siyahı (D1) and an unknown seedling (D2) that showed typical FMD symptoms and was heavily infested by fig mites were used as donor plants for attempted mite transmissions to healthy fig seedlings. Transmission electron microscopy observations of donor plant samples prior to the transmission tests were performed and showed the presence of double membrane bodies (DMBs) in the palisade mesophyll cells. Electron microscopy of all experimentally inoculated fig seedlings showed the same bodies. This result reinforced the suggestion that an agent that elicits the production of DMBs in infected cells is involved in the etiology of FMD. Double-stranded (ds)RNA analyses were also performed from experimentally inoculated plants, and dsRNAs with sizes approximately 1.30 and 1.96 kb were obtained. Reverse transcription-polymerase chain reaction (RT-PCR) products of 468 and 298 bp specific to Fig mosaic virus (FMV) were amplified from both donor and experimentally inoculated plants. BLAST analyses of nucleotide sequences of these fragments showed 90% identity with FMV for the donor plant and 94 to 96% for experimentally inoculated plants. According to these results, FMV is present in both donor and experimentally inoculated plants in Turkey, and this virus is transmissible by ''A. ficus'' from fig plant to fig plant. | ||
|AbstractCC=Yes | |AbstractCC=Yes | ||
| + | |AuthorsAbstract=No | ||
|Research topic=general biology - morphology - evolution | |Research topic=general biology - morphology - evolution | ||
|Is book=No | |Is book=No | ||
| Line 20: | Line 21: | ||
}} | }} | ||
{{Pest record | {{Pest record | ||
| − | |Pest= | + | |Pest=Emaravirus fici |
|Crop=Ficus (crop) | |Crop=Ficus (crop) | ||
|Quarantined=No | |Quarantined=No | ||
}} | }} | ||
Latest revision as of 10:24, 8 August 2023
Kadriye Çaglayan, Çigdem Ulubas Serçe, Eminur Barutçu, Kamuran Kaya, Vicente Medina, Mona Gazel, Soner Soylu and Oguzhan Çaliskan (2010)
Comparison by sequence-based and electron microscopic analyses of fig mosaic virus isolates obtained from field and experimentally inoculated fig plants
Plant Disease 94 (12), 1448-1452
Abstract: Fig mosaic disease (FMD) and the fig mite, Aceria ficus, are widespread in different fig growing provinces of Turkey. Fig trees (Ficus carica) cv. Bursa siyahı (D1) and an unknown seedling (D2) that showed typical FMD symptoms and was heavily infested by fig mites were used as donor plants for attempted mite transmissions to healthy fig seedlings. Transmission electron microscopy observations of donor plant samples prior to the transmission tests were performed and showed the presence of double membrane bodies (DMBs) in the palisade mesophyll cells. Electron microscopy of all experimentally inoculated fig seedlings showed the same bodies. This result reinforced the suggestion that an agent that elicits the production of DMBs in infected cells is involved in the etiology of FMD. Double-stranded (ds)RNA analyses were also performed from experimentally inoculated plants, and dsRNAs with sizes approximately 1.30 and 1.96 kb were obtained. Reverse transcription-polymerase chain reaction (RT-PCR) products of 468 and 298 bp specific to Fig mosaic virus (FMV) were amplified from both donor and experimentally inoculated plants. BLAST analyses of nucleotide sequences of these fragments showed 90% identity with FMV for the donor plant and 94 to 96% for experimentally inoculated plants. According to these results, FMV is present in both donor and experimentally inoculated plants in Turkey, and this virus is transmissible by A. ficus from fig plant to fig plant.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Kadriye Caglayan, Çigdem Ulubas Serçe, Soner Soylu, Vicente Medina, Mona Gazel
Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Aceria ficus | Ficus (crop) | |||
| Emaravirus fici | Ficus (crop) |
