Difference between revisions of "Microbial Ecology (2013) 65, 475-486"
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{{Publication | {{Publication | ||
| − | |Publication authors=A.S. Guidolin and F.L. Cônsoli | + | |Publication authors=A.S. Guidolin and [[Fernando L. Consoli|F.L. Cônsoli]] |
|Author Page=Fernando L. Consoli | |Author Page=Fernando L. Consoli | ||
|Publication date=2013 | |Publication date=2013 | ||
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|Publication abstract=Wolbachia is a symbiont intensively studied due to its ability to interfere with their host's reproduction, and it has been recently proposed as an alternative tool to control insect pests or vectors of diseases. The Asian citrus psyllid ''Diaphorina citri'' is an important pest of citrus since it vectors the bacterium that causes the "Huanglongbing" disease in citrus. The frequency and diversity of ''Wolbachia'' associated with ''D. citri'' is unknown, limiting the utilization of ''Wolbachia'' as an alternative strategy for insect management. Thus, we aimed to determine the natural rate of infection, to characterize the ''Wolbachia'' strains associated with this psyllid by "multilocus sequencing typing" (MLST) and wsp analysis, and to verify the association of the symbiont to particular genotypes of the host. Analysis indicated ''Wolbachia'' infects 100 % of all specimens tested from all 15 sampled populations. MLST revealed the occurrence of five new sequence types (STs) of ''Wolbachia'', while analysis based on the wsp sequences indicated only four different types of ''Wolbachia''. ST-173 was predominant, while the remaining STs were population specific. Analysis of the host-symbiont relationship did not reveal any particular association of ''Wolbachia'' and haplotypes or a decrease in nucleotide diversity of ''D. citri'' in populations in which more than one ST was recorded. The consequences of the diversity of STs reported are still unknown, but the fact that ''Wolbachia'' infection is fixed and that there is one ST with a broad distribution highlights the use of this symbiont as an alternative strategy to control ''D. citri''. | |Publication abstract=Wolbachia is a symbiont intensively studied due to its ability to interfere with their host's reproduction, and it has been recently proposed as an alternative tool to control insect pests or vectors of diseases. The Asian citrus psyllid ''Diaphorina citri'' is an important pest of citrus since it vectors the bacterium that causes the "Huanglongbing" disease in citrus. The frequency and diversity of ''Wolbachia'' associated with ''D. citri'' is unknown, limiting the utilization of ''Wolbachia'' as an alternative strategy for insect management. Thus, we aimed to determine the natural rate of infection, to characterize the ''Wolbachia'' strains associated with this psyllid by "multilocus sequencing typing" (MLST) and wsp analysis, and to verify the association of the symbiont to particular genotypes of the host. Analysis indicated ''Wolbachia'' infects 100 % of all specimens tested from all 15 sampled populations. MLST revealed the occurrence of five new sequence types (STs) of ''Wolbachia'', while analysis based on the wsp sequences indicated only four different types of ''Wolbachia''. ST-173 was predominant, while the remaining STs were population specific. Analysis of the host-symbiont relationship did not reveal any particular association of ''Wolbachia'' and haplotypes or a decrease in nucleotide diversity of ''D. citri'' in populations in which more than one ST was recorded. The consequences of the diversity of STs reported are still unknown, but the fact that ''Wolbachia'' infection is fixed and that there is one ST with a broad distribution highlights the use of this symbiont as an alternative strategy to control ''D. citri''. | ||
|AbstractCC=Yes | |AbstractCC=Yes | ||
| + | |AuthorsAbstract=No | ||
|Research topic=biocontrol - natural enemies | |Research topic=biocontrol - natural enemies | ||
|Benf research=general biology - morphology - evolution | |Benf research=general biology - morphology - evolution | ||
Latest revision as of 09:15, 24 March 2013
A.S. Guidolin and F.L. Cônsoli (2013)
Molecular characterization of Wolbachia strains associated with the invasive Asian citrus psyllid Diaphorina citri in Brazil
Microbial Ecology 65 (2), 475-486
Abstract: Wolbachia is a symbiont intensively studied due to its ability to interfere with their host's reproduction, and it has been recently proposed as an alternative tool to control insect pests or vectors of diseases. The Asian citrus psyllid Diaphorina citri is an important pest of citrus since it vectors the bacterium that causes the "Huanglongbing" disease in citrus. The frequency and diversity of Wolbachia associated with D. citri is unknown, limiting the utilization of Wolbachia as an alternative strategy for insect management. Thus, we aimed to determine the natural rate of infection, to characterize the Wolbachia strains associated with this psyllid by "multilocus sequencing typing" (MLST) and wsp analysis, and to verify the association of the symbiont to particular genotypes of the host. Analysis indicated Wolbachia infects 100 % of all specimens tested from all 15 sampled populations. MLST revealed the occurrence of five new sequence types (STs) of Wolbachia, while analysis based on the wsp sequences indicated only four different types of Wolbachia. ST-173 was predominant, while the remaining STs were population specific. Analysis of the host-symbiont relationship did not reveal any particular association of Wolbachia and haplotypes or a decrease in nucleotide diversity of D. citri in populations in which more than one ST was recorded. The consequences of the diversity of STs reported are still unknown, but the fact that Wolbachia infection is fixed and that there is one ST with a broad distribution highlights the use of this symbiont as an alternative strategy to control D. citri.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Fernando L. Consoli
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
surveys/distribution/isolation
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Diaphorina citri | ||||
| Wolbachia (genus - entomopathogens) | Diaphorina citri |
