Weed Research (2004) 44, 136-141

T. Senda, N. Kubo, M. Hirai and T. Tominaga (2004)
Development of microsatellite markers and their effectiveness in Lolium temulentum
Weed Research 44 (2), 136-141
Abstract: Screening a genomic library of Lolium temulentum (darnel) with synthetic (GT)₁₅ and (GA)₁₅ oligonucleotides, we identified microsatellite-containing clones. Based on the sequence data of the clones, specific primer pairs were synthesized and their effectiveness examined using 10 L. temulentum and two L. persicum accessions from Asia, Africa and Europe. Fifteen primer pairs amplified single fragments, and five of them showed polymorphism in polyacrylamide gel electrophoresis in the L. temulentum and L. persicum accessions. Fifteen primer pairs reported in L. perenne (perennial ryegrass) were also applied in this study. Nine of them amplified the microsatellite alleles, and two of the nine showed polymorphic alleles in L. temulentum and L. persicum. For the polymorphic markers, polymorphic information content values ranged from 0.60 to 0.86 with an average of 0.76 among 10 L. temulentum accessions. The microsatellite markers developed for L. temulentum and L. perenne should be informative and powerful in analysing the intraspecific genetic diversity and the phylogenetic relationships of L. temulentum and L. persicum.
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Link to article at publishers website
Database assignments for author(s): Tohru Tominaga

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Lolium persicum (weed)
	Lolium temulentum (weed)		Japan