Weed Biology and Management (2002) 2, 104-109

Akira Uchino and Hiroaki Watanabe (2002)
Mutations in the acetolactate synthase genes of sulfonylurea-resistant biotypes of Lindernia spp.
Weed Biology and Management 2 (2), 104-109
Abstract: Acetolactate synthase (ALS) is a key enzyme in the biosynthetic pathway of branched-chain amino acids. A mutation of the ALS gene causing amino acid substitution at the position of proline in Domain A makes ALS less sensitive to sulfonylureas, which are ALS-inhibiting herbicides. We cloned partial ALS genes from four Lindernia plants, L. dubia var. dubia, L. dubia var. major, L. micrantha and L. procumbens, for which biotypes resistant to sulfonylureas have been found in paddy fields. The clones were classified into two groups in each Lindernia plant: Als1 and Als2. Sequencing of the clones and alignment of deduced amino acid sequences with previously reported ALS of other species suggested that the cloned region contains an intron in both Als1 and Als2. Comparison of Als1 between resistant and susceptible biotypes showed that the proline of Domain A was replaced by alanine, serine or glutamine in all resistant biotypes of Lindernia plants, while it was conserved in all susceptible biotypes. This amino acid substitution in ALS encoded by Als1 is involved in the resistant mechanism of ALS to sulfonylurea in the four Lindernia plants.
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Link to article at publishers website
Database assignments for author(s): Akira Uchino

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Lindernia procumbens (weed)	Rice (Oryza)	Japan
	Lindernia dubia (weed)	Rice (Oryza)	Japan
	Lindernia micrantha (weed)	Rice (Oryza)	Japan