Viruses (2021) 13 (10 - 1952)

Annette J. Sauer, Eva Fritsch, Karin Undorf-Spahn, Kento Iwata, Regina G. Kleespies, Madoka Nakai and Johannes A. Jehle (2021)
Cross-resistance of the codling moth against different isolates of Cydia pomonella granulovirus is caused by two different but genetically linked resistance mechanisms
Viruses 13 (10 - 1952)
Abstract: Cydia pomonella granulovirus (CpGV) is a widely used biological control agent of the codling moth. Recently, however, the codling moth has developed different types of field resistance against CpGV isolates. Whereas type I resistance is Z chromosomal inherited and targeted at the viral gene pe38 of isolate CpGV-M, type II resistance is autosomal inherited and targeted against isolates CpGV-M and CpGV-S. Here, we report that mixtures of CpGV-M and CpGV-S fail to break type II resistance and is expressed at all larval stages. Budded virus (BV) injection experiments circumventing initial midgut infection provided evidence that resistance against CpGV-S is midgut-related, though fluorescence dequenching assay using rhodamine-18 labeled occlusion derived viruses (ODV) could not fully elucidate whether the receptor binding or an intracellular midgut factor is involved. From our peroral and intra-hemocoel infection experiments, we conclude that two different (but genetically linked) resistance mechanisms are responsible for type II resistance in the codling moth: resistance against CpGV-M is systemic whereas a second and/or additional resistance mechanism against CpGV-S is located in the midgut of CpR5M larvae.
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Database assignments for author(s): Regina G. Kleespies, Madoka Nakai, Johannes A. Jehle

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cydia pomonella		Germany
Betabaculovirus cypomonellae (entomopathogen)	Cydia pomonella		Germany