Viruses (2020) 12 (8 - 819)
Luciane de Nazaré Almeida dos Reis, Maria Esther de Noronha Fonseca, Simone Graça Ribeiro, Fernanda Yuri Borges Naito, Leonardo Silva Boiteux and Rita de Cássia Pereira-Carvalho (2020)
Metagenomics of neotropical single-stranded DNA viruses in tomato cultivars with and without the Ty-1 gene
Viruses 12 (8 - 819)
Abstract: A complex of begomoviruses (Geminiviridae) can cause severe tomato yield losses in the neotropics. Here, next-generation sequencing was employed for large-scale assessment of single-stranded (ss)DNA virus diversity in tomatoes either harboring or lacking the large-spectrum begomovirus tolerance Ty-1 gene. Individual leaf samples exhibiting begomovirus-like symptoms (n = 107) were field-collected, circular DNA-enriched, subdivided into pools (with and without Ty-1), and Illumina-sequenced. Virus-specific PCR and Sanger dideoxy sequencing validations confirmed 15 distinct ssDNA virus/subviral agents (occurring mainly in mixed infections), which highlight the potential drawbacks of employing virus-specific resistance in tomato breeding. More viruses (14 versus 6 species) were observed in tomatoes without the Ty-1 gene. A gemycircularvirus (Genomoviridae), a new alpha-satellite, and two novel Begomovirus species were identified exclusively in samples without the Ty-1 gene. A novel begomovirus was found only in the Ty-1 pool, being the only species associated with severe symptoms in Ty-1 plants in our survey. Our work is the first step towards the elucidation of the potential begomovirus adaptation to Ty-1 and its specific filtering effects on a subset of ssDNA viral/subviral agents.
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Database assignments for author(s): Simone G. Ribeiro, Leonardo Silva Boiteux
Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
