Virus Genes (2012) 44, 349-355

S. Gadiou, J. Ripl, B. Janourová, J. Jarosová and J.K. Kundu (2012)
Real-time PCR assay for the discrimination and quantification of wheat and barley strains of Wheat dwarf virus
Virus Genes 44 (2), 349-355
Abstract: A rapid method for detection, discrimination and quantification of wheat and barley strains of wheat dwarf virus (WDV) was successfully developed. The sensitivity of quantification of the wheat and barley strains of WDV ranged from an average of 1.2 × 10⁷-1.2 × 10² and from an average of 1.4 × 10⁷-1.4 × 10⁴ copies of viral genome, respectively. These standard serial dilutions were applied to plant and vector tissues for virus titer calculations. Both strains of WDV were clearly discriminated by specific probes and melting curve analysis. Both TaqMan^® and SYBR^® Green technologies provided accurate and reliable methods for monitoring, detection, discrimination, and quantification of WDV.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Jiban Kumar Kundu

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
surveys/sampling/distribution

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Wheat dwarf virus	Barley (Hordeum vulgare)
	Wheat dwarf virus	Wheat (Triticum)