Theoretical and Applied Genetics (2016) 129, 1507-1517

Mulualem T. Kassa, Sabrina Haas, Edgar Schliephake, Clare Lewis, Frank M. You, Curtis J. Pozniak, Ilona Krämer, Dragan Perovic, Andrew G. Sharpe, Pierre R. Fobert, Michael Koch, Ian L. Wise, Paul Fenwick, Simon Berry, James Simmonds, Delphine Hourcade, Patrice Senellart, Laure Duchalais, Olivier Robert, Jutta Förster, Julian B. Thomas, Wolfgang Friedt, Frank Ordon, Cristobal Uauy and Curt A. McCartney (2016)
A saturated SNP linkage map for the orange wheat blossom midge resistance gene Sm1
Theoretical and Applied Genetics 129 (8), 1507-1517
Abstract: Orange wheat blossom midge (OWBM, Sitodiplosis mosellana Géhin) is an important insect pest of wheat (Triticum aestivum) in many growing regions. Sm1 is the only described OWBM resistance gene and is the foundation of managing OWBM through host genetics. Sm1 was previously mapped to wheat chromosome arm 2BS relative to simple sequence repeat (SSR) markers and the dominant, sequence characterized amplified region (SCAR) marker WM1. The objectives of this research were to saturate the Sm1 region with markers, develop improved markers for marker-assisted selection (MAS), and examine the synteny between wheat, Brachypodium distachyon, and rice (Oryza sativa) in the Sm1 region. The present study mapped Sm1 in four populations relative to single nucleotide polymorphisms (SNPs), SSRs, Diversity Array Technology (DArT) markers, single strand conformation polymorphisms (SSCPs), and the SCAR WM1. Numerous high quality SNP assays were designed that mapped near Sm1. BLAST delineated the syntenic intervals in B. distachyon and rice using gene-based SNPs as query sequences. The Sm1 region in wheat was inverted relative to B. distachyon and rice, which suggests a chromosomal rearrangement within the Triticeae lineage. Seven SNPs were tested on a collection of wheat lines known to carry Sm1 and not to carry Sm1. Sm1-flanking SNPs were identified that were useful for predicting the presence or absence of Sm1 based upon haplotype. These SNPs will be a major improvement for MAS of Sm1 in wheat breeding programs.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Frank Ordon, Dragan Perovic, Edgar Schliephake, Ian L. Wise

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Sitodiplosis mosellana	Wheat (Triticum)