Theoretical and Applied Genetics (2014) 127, 1741-1751

Zhenzhong Wang, Yu Cui, Yongxing Chen, Deyun Zhang, Yong Liang, Dong Zhang, Qiuhong Wu, Jingzhong Xie, Shuhong Ouyang, Delin Li, Yinlian Huang, Ping Lu, Guoxin Wang, Meihua Yu, Shenghui Zhou, Qixin Sun and Zhiyong Liu (2014)
Comparative genetic mapping and genomic region collinearity analysis of the powdery mildew resistance gene Pm41
Theoretical and Applied Genetics 127 (8), 1741-1751
Abstract: Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici, results in large yield losses worldwide. A high-density genetic linkage map of the powdery mildew resistance gene Pm41, originating from wild emmer (Triticum turgidum var. dicoccoides) and previously mapped to the distal region of chromosome 3BL bin 0.63-1.00, was constructed using an F5:6 recombinant inbred line population derived from a cross of durum wheat cultivar Langdon and wild emmer accession IW2. By applying comparative genomics analyses, 19 polymorphic sequence-tagged site markers were developed and integrated into the Pm41 genetic linkage map. Ultimately, Pm41 was mapped in a 0.6 cM genetic interval flanked by markers XWGGC1505 and XWGGC1507, which correspond to 11.7, 19.2, and 24.9 kb orthologous genomic regions in Brachypodium, rice, and sorghum, respectively. The XWGGC1506 marker co-segregated with Pm41 and could be served as a starting point for chromosome landing and map-based cloning as well as marker-assisted selection of Pm41. Detailed comparative genomics analysis of the markers flanking the Pm41 locus in wheat and the putative orthologous genes in Brachypodium, rice, and sorghum suggests that the gene order is highly conserved between rice and sorghum. However, intra-chromosome inversions and re-arrangements are evident in the wheat and Brachypodium genomic regions, and gene duplications are also present in the orthologous genomic regions of Pm41 in wheat, indicating that the Brachypodium gene model can provide more useful information for wheat marker development.
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Link to article at publishers website
Database assignments for author(s): Qixin Sun

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Blumeria graminis	Wheat (Triticum)