Theoretical and Applied Genetics (2004) 108, 651-656

S.A. Mehlenbacher, R.N. Brown, J.W. Davis, H. Chen, N.V. Bassil, D.C. Smith and T.L. Kubisiak (2004)
RAPD markers linked to eastern filbert blight resistance in Corylus avellana
Theoretical and Applied Genetics 108 (4), 651-656
Abstract: A total of 1,110 decamer primers were screened for RAPD markers linked to a dominant allele in hazelnut (Corylus avellana) that confers resistance to eastern filbert blight caused by Anisogramma anomala. Twenty RAPD markers linked in coupling, and five markers linked in repulsion, were found. A seedling population was used to construct a linkage map of the region flanking the resistance locus. The map spans 46.6 cM, with 14 markers on one side of the resistance locus and eight on the other side. Eleven markers showed less than 3% recombination with resistance, including three that showed no recombination. Seven of these 11 markers are sufficiently robust to allow their use in marker-assisted selection. These include AA12₈₅₀ which shows no recombination, and six markers on one side of the resistance locus: 173₅₀₀, 152₈₀₀, 122₈₂₅, 275₁₁₃₀, H19₆₅₀ and O16₁₂₅₀. Marker 268₅₈₀, which flanks the resistance locus on the other side, is also suitable for use in marker-assisted selection, but shows 5.8% recombination with resistance. Other markers are less suitable for marker-assisted selection because of sensitivity to changes in primer or MgCl₂ concentration, or the long time required for electrophoresis to separate bands of similar size. The 16 markers closest to the resistance locus were cloned and sequenced. The W07₃₆₅ marker, which showed no recombination with the resistance locus but is difficult to score, includes a CT microsatellite repeat. The sequence information will allow the design of SCAR primers and eventual map-based cloning of the resistance allele.
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Link to article at publishers website
Database assignments for author(s): Thomas L. Kubisiak

Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Anisogramma anomala	Hazelnut (Corylus)