The Journal of Eukaryotic Microbiology (2005) 52, 55-60

Kildare Miranda, Anibal E. Vercesi, Rosana Catisti, Wanderley De Souza, Claudia O. Rodrigues and Roberto Docampo (2005)
P-type proton ATPases are involved in intracellular calcium and proton uptake in the plant parasite Phytomonas francai
The Journal of Eukaryotic Microbiology 52 (1), 55-60
Abstract: The use of digitonin to permeabilize the plasma membrane of promastigotes of Phytomonas francai allowed the identification of two non-mitochondrial Ca²⁺ compartments; one sensitive to ionomycin and vanadate (neutral or alkaline), possibly the endoplasmic reticulum, and another sensitive to the combination of nigericin plus ionomycin (acidic), possibly the acidocalcisomes. A P-type (phospho-intermediate form) Ca²⁺-ATPase activity was found to be responsible for intracellular Ca²⁺ transport in these cells, with no evidence of a mitochondrial Ca²⁺ transport activity. ATP-driven acidification of internal compartments in cell lysates and cells mechanically permeabilized was assayed spectrophotometrically with acridine orange. This activity was inhibited by low concentrations of vanadate and digitonin, was insensitive to bafilomycin A₁, and stimulated by Na⁺ ions. Taken together, our results indicate that P-type ATPases are involved in intracellular Ca²⁺ and H⁺ transport in promastigotes of P. francai.
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Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytomonas francai