Scientia Agricola (2007) 64, 641-644

Ana Cristina Fermino Soares, Carla da Silva Sousa, Marlon da Silva Garrido and Jane Oliveira Perez (2007)
Production of streptomycete inoculum in sterilized rice
Scientia Agricola 64 (6), 641-644
Abstract: Actinomycetes are important plant disease control and growth promotion agents, which makes it necessary to develop technology to produce large quantities of inoculum for green-house and field work. The present study had the objective of evaluating the growth of several isolates of Streptomyces in sterile rice for inoculum production. The sterile rice was inoculated with isolates of S. thermotolerans, S. griseus subsp. griseus, Streptomyces sp. N0035, S. purpurascens, and Streptomyces sp., and incubated at 28 ± 2ºC. Five days after its inoculation, mycelial growth and sporulation was observed for all Streptomyces isolates on the rice grains. Twelve days after incubation, the colonized rice was transferred to envelopes of dark brown paper and let to dry in an incubator at 30ºC for three days. After drying, 1g of colonized rice was added to 200 mL of sterile distilled water and the number of spores was counted under a microscope with a Newbauer counting chamber. Spore production varied from 0.14 × 10⁹ to 1.47 × 10⁹ spores per gram of rice and differed among the Streptomyces species. Sterile rice can be an alternative substrate for low cost mass production of Streptomyces inoculum.
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Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
rearing/culturing/mass production

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Meloidogyne incognita
Streptomyces griseus (antagonist)	Meloidogyne incognita
Streptomyces purpurascens (antagonist)