REDIA (2008) 91, 181-183

E. Cosi, O. Triggiani, T. Irdani, E. Tarasco and P.F. Roversi (2008)
First results of entomopathogenic nematodes cryopreservation in liquid nitrogen and storage at -140°C
REDIA 91, 181-183
Abstract: Entomopathogenic nematodes (EPN) are using for biological control in insect pests management. Cryopreservation of nematodes is an established technique to preserve strains in both research and commercial practice. We tested two different vitrification protocols on the Apulian strain of Steinernema carpocapsae (Weiser) ItS-MR7. After liquid nitrogen quenching, the storage of the vitrified EPN was carried out in a mechanical freezer at the temperature of -140°C. We showed that our cold storage method, joined with a protocol previuosly described by BAI et al. (2004), could maintain a high survival rate and high reproduction capability of the thawed nematodes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Pio Federico Roversi

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
formulation/storage of bioagents

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Steinernema carpocapsae/Xenorhabdus nematophila (entomopathogen)