Plant Protection Science (2000) 36, 46-52

B. Kokoskova, I. Pankova and V. Krejzar (2000)
Characteristics of polyclonal antisera for detection and determination of Clavibacter michiganensis subsp. insidiosus
Plant Protection Science 36 (2), 46-52
Abstract: Three polyclonal antisera for detection and determination of Clavibacter michiganensis subsp. insidiosus, the causal agent of bacterial wilt of lucerne, were prepared. The original titers of these antisera CI 12, CI 13 and CI 14 were 1:32, 1:256 and 1:64, respectively. Their specificity was increased after cross-absorption with Ralstonia solanacearum and Pseudomonas fluorescens, after which the titers of antisera CI 12, CI 13 and CI 14 decreased to 1:16, 1:128 and 1:8, respectively. Polyclonal antisera Cl 12 and Cl 13 could be used for determination and detection of C. m. insidiosus by the ELISA method. Using PTA ELISA, a homologous antigen was identified at a concentration of 10⁶-10⁵ cfu/ml, while with DAS ELISA only at one of 10⁷-10⁶ cfu/ml. Highly fluidal strains of C. m. insidiosus were not reliably identified by the polyclonal antisera. The PTA ELISA test was suitable for rapid negative diagnosis.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Iveta Pánková, Blanka Kokoskova

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Clavibacter insidiosus	Alfalfa/lucerne (Medicago sativa)