Plant Pathology (2010) 59, 348-357

M.A. Dita, C. Waalwijk, I.W. Buddenhagen, M.T. Souza Jr. and G.H.J. Kema (2010)
A molecular diagnostic for tropical race 4 of the banana fusarium wilt pathogen
Plant Pathology 59 (2), 348-357
Abstract: This study analysed genomic variation of the translation elongation factor 1-alpha (TEF-1-alpha) and the intergenic spacer region (IGS) of the nuclear ribosomal operon of Fusarium oxysporum f. sp. cubense (Foc) isolates, from different banana production areas, representing strains within the known races, comprising 20 vegetative compatibility groups (VCG). Based on two single nucleotide polymorphisms present in the IGS region, a PCR-based diagnostic tool was developed to specifically detect isolates from VCG 01213, also called tropical race 4 (TR4), which is currently a major concern in global banana production. Validation involved TR4 isolates, as well as Foc isolates from 19 other VCGs, other fungal plant pathogens and DNA samples from infected tissues of the Cavendish banana cultivar Grand Naine (AAA). Subsequently, a multiplex PCR was developed for fungal or plant samples that also discriminated Musa acuminata and M. balbisiana genotypes. It was concluded that this diagnostic procedure is currently the best option for the rapid and reliable detection and monitoring of TR4 to support eradication and quarantine strategies.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Miguel Angel Dita, Gert H.J. Kema, Cees Waalwijk

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Fusarium odoratissimum	Banana/plantain (Musa)