Plant Pathology (2009) 58, 436-442

J.A. Herrera-Vásquez, M.C. Córdoba-Sellés, M.C. Cebrián, A. Alfaro-Fernández and C. Jordá (2009)
Seed transmission of melon necrotic spot virus and efficacy of seed-disinfection treatments
Plant Pathology 58 (3), 436-442
Abstract: Rates of seed transmission of Melon necrotic spot virus (MNSV) were estimated in seedlings grown from commercial melon (Cucumis melo) cv. Galia F₁ seeds. Seedlings at the cotyledon stage and adult plants were assayed for MNSV by DAS-ELISA and RT-PCR. None of the seedling groups tested positive for MNSV by ELISA. The proportion of seedlings infected with MNSV was at least 7 and 8% in seed lots 05 and 06, respectively, as estimated from RT-PCR analysis of grouped seedlings. Fourteen and eight grouped samples (10 seedlings per group), of a total of 200 and 100 seedlings, respectively, grown from infected seeds were MNSV-positive in seed lots 05 and 06, respectively, corresponding to seed-to-seedling transmission rates of 11·3 and 14·8%, respectively. Several seed-disinfection treatments were evaluated for their ability to prevent seed transmission of MNSV. The results suggest that a treatment of 144 h at 70°C can be used to eradicate MNSV in melon seeds without hindering germination.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): José Angel Herrera-Vasquez, Ana Alfaro-Fernandez, Maria del Carmen Córdoba-Sellés, Concepcion Jorda

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Gammacarmovirus melonis	Melon (Cucumis melo)