Plant Pathology (1999) 48, 235-239

Arie Rosner, L. Maslenin and S. Spiegel (1999)
Double-stranded conformation polymorphism of heterologous RNA transcripts and its use for virus strain differentiation
Plant Pathology 48 (2), 235-239
Abstract: Polymorphism of heterologous duplexes of RNA transcripts of PNRSV isolates was demonstrated. Complementary RNA trancripts were copied from PCR products of PNRSV isolates, to which the T7 RNA promoter was added on either the 3' or 5'-ends. Heterologous duplexes made from complementary RNA transcripts originating from several virus isolates differed in their electrophoretic mobilities. This polymorphism is hypothesized to be conferred by conformational variations among the transcript duplexes, and was termed by us 'double-stranded transcript conformation polymorphism (ds-TCP)'. This method may serve as an additional tool in virus strain differentiation.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Prunus necrotic ringspot virus		Israel