Plant Disease (2022) 106, 165-173

Hafiz Muhammad Usman, Qin Tan, Fei Fan, Mohammad Mazharul Karim, Wei-Xiao Yin, Fu-Xing Zhu and Chao-Xi Luo (2022)
Sensitivity of Colletotrichum nymphaeae to six fungicides and characterization of fludioxonil-resistant isolates in China
Plant Disease 106 (1), 165-173
Abstract: Colletotrichum nymphaeae is the dominant species causing anthracnose disease of peach in China. In this study, 140 isolates of C. nymphaeae were assessed for their sensitivity to six fungicides. It was found that C. nymphaeae was highly resistant to carbendazim, procymidone, and boscalid but sensitive to pyraclostrobin and prochloraz. For fludioxonil, the fungus exhibited differential sensitivities (i.e., approximately 14% of isolates were resistant to fludioxonil and the resistance was stable). Fludioxonil-resistant isolates had a mean EC₅₀ value of 2.2380 µg/ml, whereas the mean EC₅₀ value was 0.0194 µg/ml in fludioxonil-sensitive isolates. The mean EC₅₀ values of C. nymphaeae for pyraclostrobin and prochloraz were 0.0083 µg/ml and 0.002 µg/ml, respectively. No cross-resistance was observed between fungicides from different groups. Mycelial growth rate, control efficacy, and osmotic stress responses were significantly different (P < 0.05) between fludioxonil-sensitive (Flu^S) and -resistant (Flu^R) isolates, but no significant difference was observed (P > 0.05) in virulence and sporulation between Flu^S and Flu^R isolates. No mutation was detected in coding regions of the CnOs-1, Cal, Hk1, Hog1, TPI, and Mrr1 genes. Interestingly, with fludioxonil treatment, the expression of ABC transporter gene atrB was significantly overexpressed in some resistant isolates. However, overexpression of the atrB gene was not detected in one moderately and one highly resistant isolate, indicating that other unknown mechanisms may be involved. Current findings uncovered several effective chemicals and provided the foundation for designing management strategies to practically control peach anthracnose with the most effective demethylation inhibitor fungicides and quinone outside inhibitor fungicides.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Chao-Xi Luo

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Colletotrichum nymphaeae	Peach/nectarine (Prunus persica)