Plant Disease (2006) 90, 723-728

C. Vaïanopoulos, A. Legrève, C. Lorca, V. Moreau, S. Steyer, H. Maraite and C. Bragard (2006)
Widespread occurrence of Wheat spindle streak mosaic virus in Belgium
Plant Disease 90 (6), 723-728
Abstract: In order to assess the occurrence of Wheat spindle streak mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reaction (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Germany, Italy, and the United States. The primers also were designed for virus quantification by real-time RT-PCR with SYBR-Green. No cross-reaction with soilborne cereal viruses such as Barley mild mosaic virus, Barley yellow mosaic virus, Soil-borne cereal mosaic virus, and Soil-borne wheat mosaic virus was observed. The RT-PCR and real-time quantitative RT-PCR allowed a more sensitive detection of WSSMV than enzyme-linked immunosorbent assay. The incidence of WSSMV in Belgium was evaluated using a bioassay with wheat cvs. Cezanne and Savannah and rye cv. Halo, grown in 104 Belgian soils. The presence of WSSMV was detected from plants grown in 32% of the soils. The RT-PCR methods developed here, combined with large sampling, allowed WSSMV to be detected for the first time in Belgium. The real-time quantitative RT-PCR was developed as a tool for evaluating the resistance to WSSMV by quantifying the virus concentration in wheat cultivars.
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Database assignments for author(s): Claude Bragard, Anne Legrève

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Wheat spindle streak mosaic virus		France
	Wheat spindle streak mosaic virus		Germany
	Wheat spindle streak mosaic virus		Italy
	Wheat spindle streak mosaic virus	Rye (Secale cereale)	Belgium
	Wheat spindle streak mosaic virus	Wheat (Triticum)	Belgium