Plant Disease (1997) 81, 1231-1235
M. Ravelonandro, R. Scorza, J.C. Bachelier, G. Labonne, L. Levy, V. Damsteegt, A.M. Callahan and J. Dunez (1997)
Resistance of transgenic Prunus domestica to Plum pox virus infection
Plant Disease 81 (11), 1231-1235
Abstract: Transgenic plum trees (Prunus domestica) containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored by evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations and analyses over 3 years including two dormancy cycles, one out of five transgenic clones (C-5), was found to be resistant to infection whether inoculated by aphids or by chip budding. PPV could not be detected in any inoculated plants of the C-5 clone by immunoblot or immunocapture-reverse transcriptase-polymerase chain reaction assays. To our knowledge, this is the first P. domestica clone resistant to PPV infection produced by genetic engineering.
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Database assignments for author(s): Vernon D. Damsteegt, Laurene Levy
Research topic(s) for pests/diseases/weeds:
resistance/tolerance/defence of host
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Plum pox virus | Plum/prune (Prunus domestica) |