Phytopathology (2014) 104, 804-811

Gabriela R. Teresani, Edson Bertolini, Ana Alfaro-Fernández, Carmen Martínez, Francisco André Ossamu Tanaka, Elliot W. Kitajima, Montserrat Roselló, Susana Sanjuán, Juan Carlos Ferrándiz, María M. López, Mariano Cambra and María Isabel Fontjava (2014)
Association of ' Candidatus Liberibacter solanacearum' with a vegetative disorder of celery in Spain and development of a real-time PCR method for its detection
Phytopathology 104 (8), 804-811
Abstract: A new symptomatology was observed in celery (Apium graveolens) in Villena, Spain in 2008. Symptomatology included an abnormal amount of shoots per plant and curled stems. These vegetative disorders were associated with ' Candidatus Liberibacter solanacearum' and not with phytoplasmas. Samples from plant sap were immobilized on membranes based on the spot procedure and tested using a newly developed real-time polymerase chain reaction assay to detect ' Ca. L. solanacearum'. Then, a test kit was developed and validated by intralaboratory assays with an accuracy of 100%. Bacterial-like cells with typical morphology of ' Ca. Liberibacter' were observed using electron microscopy in celery plant tissues. A fifth haplotype of ' Ca. L. solanacearum', named E, was identified in celery and in carrot after analyzing partial sequences of 16S and 50S ribosomal RNA genes. From our results, celery (family Apiaceae) can be listed as a new natural host of this emerging bacterium.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Ana Alfaro-Fernandez, Elliot W. Kitajima, Maria M. Lopez

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Liberibacter solanacearum	Carrot (Daucus carota)	Spain (continental)
	Liberibacter solanacearum	Celery (Apium graveolens)	Spain (continental)