Phytopathology (2004) 94, 69-75

Susana Llamas, Claudio Sandoval, Mar Babin, Judy Pogany, Jozef J. Bujarski and Javier Romero (2004)
Effect of the host and temperature on the formation of defective RNAs associated with Broad bean mottle virus infection
Phytopathology 94 (1), 69-75
Abstract: Previously, we demonstrated that Broad bean mottle virus (BBMV), a member of the genus Bromovirus, could accumulate RNA 2-derived defective interfering (DI) RNAs during infection. In this work, we study how host and environmental factors affect the accumulation of DI RNAs. Serial passages of BBMV through selected plant species reveal that, with low-multiplicity inocula, some systemic hosts (Vicia faba, Nicotiana clevelandii, and N. tabacum cv. Samsum) support DI RNA accumulation after the first passage cycle but other hosts (Phaseolus vulgaris, Pisum sativum, and Glycine max) do not. However, several passages with the high-multiplicity inocula can generate DI RNAs in pea plants. Local lesion hosts (Chenopodium quinoa, C. amaranticolor, and C. murale) remain free of the DI RNA components. The size of the de novo-formed DI RNAs depends on the host and on environmental conditions. For instance, broad bean plants cultivated in a greenhouse or in a growth chamber at 20°C accumulated DI RNAs of 2.4 or 1.9 kb in size, respectively. A reverse trend was observed in pea plants. Lower temperatures greatly facilitated the formation of DI RNAs in broad bean and pea hosts after the first passage. The importance of these findings for the studies on DI RNAs are discussed.
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Database assignments for author(s): Jozef J. Bujarski, Javier Romero

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Broad bean mottle virus	Soybean (Glycine max)
	Broad bean mottle virus	Tobacco (Nicotiana tabacum)
	Broad bean mottle virus	Pea (Pisum sativum)
	Broad bean mottle virus	Beans (Phaseolus)
	Broad bean mottle virus	Broad/faba bean (Vicia faba)