Phytopathology (1996) 86, p. S73 (Wang et al.)

R.Y. Wang and T.P. Pirone (1996)
Relationship between poly-L-ornithine mediated transmission of Tobacco mosaic virus by Myzus persicae and retention of virions in the stylets
Phytopathology 86 (11 suppl.), S73-S73
Abstract: Light microscopic autoradiography was used to test the hypothesis that poly-L-omithine acts by allowing retention of TMV in aphid stylets, similar to the way in which helper component acts to mediate the aphid transmission of potyviruses. Myzus persicae consistently transmitted PLO-treated TMV at a low level but did not transmit untreated TMV Uptake and stylet retention of PLO-treated and untreated TMV were compared by feeding aphids ¹²⁵I-labeled virions, followed by liquid scintillation counting and autoradiography. No differences were found in the overall amount of label in the bodies of aphids fed on PLO-treated or untreated ¹²⁵I-labeled TMV by liquid scintillation counting. When aphids acquired PLO-treated ¹²⁵I-labeled TMV, 43.3% of 157 aphids retained label in the stylets whereas only 0.5% of 197 aphids retained non-PLO-treated ¹²⁵I-labeled TMV as determined by autoradiography. Therefore, retention of virions in aphid stylets appears to be a prerequisite for the transmission of TMV by aphids.

Research topic(s) for pests/diseases/weeds:
transmission/dispersal of plant diseases

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Myzus persicae
	Tobacco mosaic virus