Phytopathology (1996) 86, p. S72 (Yang et al.)

T.C. Yang, F.W. Zetter and J.E. Polston (1996)
Direct tissue blotting (DTB) used to detect lily X potexvirus (LVX)
Phytopathology 86 (11 suppl.), S72-S72
Abstract: LVX polyclonal antiserum from the Flower Bulb Inspection Service (Lisse, The Netherlands) at a dilution of 1:1000 was used in DTB tests as described by Lin et al. (Phytopathol. 80:824) to detect LVX in fully developed Asiatic and Oriental lily (Lilium spp.) bulbs. LVX was detected in all bulb scales, roots, and basal plate tissue tested. 55 of 101 Stargazer, 3 of 4 Laura Lee, and 2 of 3 Sirocco bulbs were infected with LVX. These bulbs were also doubly infected with lily symptomless, cucumber mosaic, or tulip breaking viruses. ELISA tests were used to confirm the presence of all four viruses. In indirect gold immunolabeling tests with LVX antiserum (1:1000), only LVX virions (ca. 550 nm long) were decorated with 10 nm colloidal gold particles. Virions ca. 650 nm long were also present but did not become labeled. DTB appears to be a reliable alternative to other methods for detecting LVX.

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Lily virus X	Lilium (crop)