Phytopathology (1996) 86, p. S15 (Hatziloukas et al.)

E. Hatziloukas and N.W. Schaad (1996)
Atypical genetic structure of phaseolotoxin produced by a nonphaseolicola Pseudomonas syringae pathovar
Phytopathology 86 (11 suppl.), S15-S15
Abstract: The bacterium Pseudomonas syringae pv. phaseolicola (PSP), the causative agent of halo blight of bean, is the only confirmed producer of phaseolotoxin. Phaseolotoxin production has been a major taxonomic criterion for the classification of PSP. Detection by PCR of a DNA segment involved in phaseolotoxin biosynthesis and in vitro toxin production by a bacterium isolated from the field in France, which was identified as Pseudomonas syringae pv. syringae (PSS; Tourte and Manceau, 1,995, Europ. J. Plant Path., 101:483-490), suggests a horizontal transfer of the tox-argK cluster. Such a phenomenon has important taxonomic, phytosanitary, and genetic implications. We tested this strain (CFBP3388) along with PSP and PSS with several PCR primers that amplify different regions of the PS genomes, including the 1) tox-argK and hip clusters, 2) the rDNA region, and 3) the rcsA gene. Preliminary results reveal that the genetic structure of the tox-argK cluster from CFBP3388, differs in at least one region from the archetypal from PSP. Based on the PCR analyses of the other genomic regions, CFBP3388 differs from both PSP and PSS.

Research topic(s) for pests/diseases/weeds:
identification/taxonomy
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Pseudomonas syringae pv. syringae		France
	Pseudomonas syringae