Phytopathology (1996) 86, p. S11 (Pan et al.)

Y.-B. Pan, M.P. Grisham and D.M. Burner (1996)
Detection and identification of Xanthomonas albilineans, the sugarcane leaf scald pathogen, by polymerase chain reaction
Phytopathology 86 (11 suppl.), S11-S11
Abstract: A practical PCR procedure was developed for the detection and identification of Xanthomonas albilineans. The primers, Ala4 (5' CCC GAC TGG CTC CAC CAC TG 3') and L1 (5' CAA GGC ATC CAC CGT 3'), target the intergenic transcribed spacer (ITS) region between the 16S and 23S rRNA genes. The procedure takes about six hours to run with a material cost of less than $2 per sample. A 360 bp DNA product was amplified from all Xa strains tested including six strains known to belong to either serovar I (G44 and CP5), II (HV5 and C1-35), or III (G7 and STK3). DNA products of different sizes were also amplified from other Xanthomonads of non-sugarcane origin and from unidentified non-Xa saprophytes from sugarcane. Among these non-Xa saprophytes of sugarcane at least four different groups were identified based on the sizes of their PCR products. Their pathogenicity on sugarcane is under investigation. Following this PCR procedure, Xanthomonas albilineans was detected from asymptomatic leaf diffusate and sap samples obtained from commercial fields.
Database assignments for author(s): Yong-Bao Pan

Research topic(s) for pests/diseases/weeds:
identification/taxonomy

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Xanthomonas albilineans	Sugarcane (Saccharum officinarum)