Phytopathology (1981) 71, 1104-1107

Guido Boccardo, R.G. Beaver, J.W. Randles and Julita S. Imperial (1981)
Tinangaja and bristle top, coconut diseases of uncertain etiology in Guam, and their relationship to Cadang-Cadang disease of coconut in the Philippines
Phytopathology 71 (10), 1104-1107
Abstract: The symptomatologies of tinangaja and bristle top, two disorders afflicting coconut palms on the island of Guam, were compared with cadang-cadang disease in the Philippines. Nucleic acids were extracted from leaf samples from healthy and diseased palms through a procedure involving precipitation with polyethylene glycol, phenol and chloroform extractions, fractionation with 2 M LiCl, and further purification with cetyltrimethylammonium bromide. Comparative electrophoretic analysis established that two low-molecular-weight RNAs with the same apparent mobilities in 5% polyacrylamide gels as the diagnostic viroidlike RNAs (ccRNA-1 and ccRNA-2) associated with cadang-cadang disease were correlated uniquely with tinangaja symptoms. A ³H-labeled DNA probe complementary to ccRNA-1 (cDNA) was used to show that the tinangaja-related RNAs have nucleotide sequences equivalent to ccRNA-1. Tinangaja is therefore considered to have the same etiology as cadang-cadang, a disease formerly believed to be restricted to the Philippines. Nucleic acids extracted from coconuts affected with bristle top did not contain such viroidlike RNAs.
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Database assignments for author(s): John W. Randles, Guido Boccardo

Research topic(s) for pests/diseases/weeds:
environment - cropping system/rotation

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Coconut tinangaja viroid	Coconut (Cocos nucifera)	Guam