Phytopathologia Mediterranea (2016) 55, 401-409

Eleonora Satta, Ana Sofia Ramirez, Samanta Paltrinieri, Nicoletta Contaldo, Purifacation Benito, José Bismark Poveda and Assunta Bertaccini (2016)
Simultaneous detection of mixed ' Candidatus Phytoplasma asteris' and ' Ca. Liberibacter solanacearum' infection in carrot
Phytopathologia Mediterranea 55 (3), 401-409
Abstract: Symptoms of shoot and root malformation were observed in carrot plants in fields located in the North of Gran Canaria Island (Spain), during surveys carried out in spring 2015 and 2016. Total DNA extraction from the leaves of symptomatic plants was performed and PCR assays were carried out to detect the agents possibly associated to the observed symptoms. While ' Candidatus Liberibacter solanacearum' was detected in the majority of tested symptomatic samples, phytoplasmas belonging to the ribosomal group 16SrI were detected only in two samples. Phylogenetic analyses and sequencing, together with virtual RFLP, confirmed that ' Ca. L. solanacearum' strains detected in carrot samples belong to haplotype D.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Full text of article
Database assignments for author(s): Assunta Bertaccini, Nicoletta Contaldo

Research topic(s) for pests/diseases/weeds:
surveys/sampling/distribution
identification/taxonomy

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Phytoplasma asteris	Carrot (Daucus carota)	Spain (Canary Is.)
	Liberibacter solanacearum	Carrot (Daucus carota)	Spain (Canary Is.)