Phytopathologia Mediterranea (2007) 46, 195-200

Samar Misbeh, Abdullah Al-Musa, Ghandi Anfoka and Nida Salem (2007)
Molecular characterization of Grapevine virus A isolates from Jordan
Phytopathologia Mediterranea 46 (2), 195-200
Abstract: A total of 1141 samples of petioles and canes of grapevines from different locations in Jordan were tested by DAS-ELISA for Grapevine virus A (GVA). About 14.2% of samples were infected with GVA. Using Reverse Transcription- Polymerase Chain Reaction (RT-PCR) or Immunocapture (IC)-RT-PCR, a fragment of 430 bp of the coat protein gene was amplified using the primer pair H587/C995. Fifteen clones were sequenced and aligned with each other and with the Jordanian isolate (GVA-Jo) using the DNAMAN program. Alignment analysis showed that all Jordanian isolates shared 100% nucleotide identity with each other and with the Jordanian isolate GVA-Jo. To classify the collected GVA isolates in one of the GVA groups a pairwise nucleotide alignment between isolate GVA-5R and isolates from South Africa; 92/778, JP98 and P163-1 representing GVA group I, II and III respectively was done. Alignment results indicated that isolate GVA-5R shared 90, 83, and 76% nucleotide similarity with GVA groups I, II, and III, respectively.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Samar Misbeh, Abdullah Al-Musa, Ghandi H. Anfoka

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Grapevine virus A	Grapevine (Vitis)	Jordan