Phytoparasitica (2011) 39, 497-502

S.K. Raj, S.K. Snehi, M.S. Khan, A.K. Tiwari and G.P. Rao (2011)
Molecular identification of an Ageratum enation virus isolate associated with mosaic disease of pointed gourd (Trichosanthes dioica) in India
Phytoparasitica 39 (5), 497-502
Abstract: A severe mosaic disease of pointed gourd (Trichosanthes dioica Roxb.) was observed with significant disease incidence in Gopalganj, India, during 2008. Begomovirus was detected from symptomatic leaf samples by polymerase chain reaction (PCR) using coat protein gene-specific primers of a well characterized begomovirus which revealed positive amplification of expected size ~800 bp DNA band. To confirm begomovirus association, the complete DNA-A was amplified using three sets of begomovirus DNA-A primers. The amplicons were cloned, sequenced, and sequence of the complete DNA-A (2757 nt) was determined by combining the sequence data of all amplicons (Accession no. GQ268327). The sequence data showed 99-93% sequence identities and close phylogenetic relationships with isolates of Ageratum enation virus (AgEV). The begomovirus associated with mosaic disease of T. dioica was identified as an isolate of Ageratum enation virus, which is a new record from India.
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Link to article at publishers website
Database assignments for author(s): Shri Krishna Raj, Ajay Kumar Tiwari, Govind P. Rao

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Ageratum enation virus	Trichosanthes (crop)	India