Phytoparasitica (2008) 36, 220-230
S. Subramanian, R.J. Rabindra and S. Sithanantham (2008)
Genetic and biological variations among Plutella xylostella granulovirus isolates
Phytoparasitica 36 (3), 220-230
Abstract: Success of a baculovirus in the field depends on the selection and utilization of effective isolates of viruses with increased pathogenicity and virulence. Isolates of Plutella xylostella granuloviruses collected from Kenya and India were compared for genetic variations by restriction endonuclease enzyme analysis and for biological activity based on their median lethal concentration and time-to-kill early- and late-third instars. The P. xylostella granulovirus isolates in Kenya were more diverse than those in India and were considerably different from the latter, with only 53 - 61% similarity. The Kenyan and Indian isolates did not differ significantly for their median lethal concentration, but the Kenyan isolate collected from Kibwezi was significantly faster than other isolates in killing early- and latethird instar P. xylostella. Cluster analysis of restriction fragment marker profiles of the P. xylostella granulovirus with other granuloviruses revealed that it grouped into a cluster with Spodoptera litura granulovirus, whereas Helicoverpa armigera and Chilo infuscatellus granuloviruses grouped into another cluster. Results of the present study indicate the existence of genetically and biologically diverse isolates of P. xylostella granulovirus which could be further developed into effective biopesticides for the management of diamondback moth.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website
Database assignments for author(s): Sevgan Subramanian
Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
general biology - morphology - evolution
molecular biology - genes
Pest and/or beneficial records:
| Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
|---|---|---|---|---|
| Plutella xylostella | India | |||
| Plutella xylostella | Kenya | |||
| Betabaculovirus pluxylostellae (entomopathogen) | Plutella xylostella | India | ||
| Betabaculovirus pluxylostellae (entomopathogen) | Plutella xylostella | Kenya |
