Phytoparasitica (2003) 31, p. 420 (Korolev et al.)

Nadia Korolev, Y. Elad and Talma Katan (2003)
Mycelial interaction among Botrytis cinerea strains tested by heterokaryon formation or barrage phenomenon
Phytoparasitica 31 (4), 420-420
Proceedings of the 24th Congress of the Israeli Phytopathological Society
Abstract: A total of 21 B. cinerea strains originating from fieve host plants at ten sites throughout Israel, were tested for mycelial interaction. Complementation between auxotrophic selenate-resistant (sul) mutants, and antagonism (barrage) between wild-type strains served as criteria for compatibility: Complementation indicates that the parent strains are compatible, whereas barrage indicates that the strains are incompatible. sul mutants resistant or sensitive to chromate were paired on the Botrytis Minimal Medium. Wild-type growth of complementary heterokaryons was always observed between different mutant phenotypes derived from the same parent, as well as in some inter-strain pairings. Most isolates were placed into the same group based either on positive complementation reaction between their mutants or, more often, on positive reaction with mutants of 'bridging' strains, compatible with two or more isolates that were not compatible directly. Barrage between wild-type isolates, in the form of a zone of sparse mycelium with or without dark pigmentation of the agar along the line of confrontation, was observed in ~ 35% of pairings. Although isolates were often incompatible with each other, all of them were eventually placed into one group based on their compatibility with a common third strain ('bridging'), confirming the data of heterokaryon compatibility by sul mutants. sul mutants were pathogenic to bean and strawberry and could be used as marked strains in epidemiological studies.
Database assignments for author(s): Nadia Korolev, Yigal Elad

Research topic(s) for pests/diseases/weeds:
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Botrytis cinerea	Strawberry (Fragaria)	Israel
	Botrytis cinerea	Beans (Phaseolus)	Israel