Phytoparasitica (2002) 30 (3) - Characterization of ...

Merav Bar-Shimon, Hila Yehuda, Lea Cohen, Batya Weiss, Alexandra Kobeshnikov, M. Goldway and S. Droby (2002)
Characterization of extracellular lytic enzymes produced by the postharvest biocontrol agent Candida oleophila
Phytoparasitica 30 (3)
The 23rd Congress of the Israeli Phytopathological Society - February 11-12, 2002
Abstract: The enhancement of biocontrol activity of biocontrol agents may be the most important factor in their success in controlling postharvest fruit diseases. In our efforts to identify genetic traits of the yeast Candida oleophila and to determine their potential to enhance its biocontrol activity, we have been studying the yeast's ability to produce fungal cell-wall-degrading enzymes. This study focused on four enzymes secreted by C. oleophila: exo-glucanase, endo-glucanase, chitinase and protease. The influence of the type of growth and carbon sources on the production of these enzymes by the yeast antagonist was determined using calorimetric assays and plates containing the septic substrates. SDS-PAGE and activity gels of partially purified enzyme preparations were used to study the isozyme profiles. In addition, a full length gene of secreted ß-1,3-glucanase of this yeast (CoEXG1) was cloned and analyzed. Sequencing analysis revealed that CoEXG1 carries the signature pattern of family 5-glycohydrolase, has a putative secretion leader, and has a high degree of identity to other yeast ß-1,3-glucanase. C. oleophila transformants harboring two copies of the CoEXG1 and its putative promoter region exhibited increased production (at least two-fold) of ß-1,3-glucanase. Cloning and sequence analysis of chitinase genes from C. oleophila are under way.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)

Research topic(s) for pests/diseases/weeds:
biocontrol - natural enemies
Research topic(s) for beneficials or antagonists:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
Candida oleophila (antagonist)