Phytoparasitica (1998) 26 (1) - Host effect on accumulation ...

Javier Romero, Antonio Molina-Garcia, Mar Babin, Judy Pogany and Jozef J. Bujarski (1998)
Host effect on accumulation and encapsidation of defective-interfering RNAs in Broad bean mottle virus (BBMV) infections
Phytoparasitica 26 (1)
Xth International Congress of Virology, August 11-16, 1996, Binyanei haOoma, Jerusalem, Israel, poster
Abstract: BBMV encapsidates defective-interfering (DI) components that are formed by in-frame deletions within the RNA 2 openreading frame. The biological characteristics of these DI RNAs include exacerbation of the severity of symptoms on certain hosts and the lack of DI RNA through other hosts. In order to study the molecular aspects of the relationship between DI RNA transmission and symptoms, we investigated the DI RNA accumulation and encapsidation in various local lesion or systemic host plants. This was analyzed using Northern blots and RT-PCR of total and virion RNA preparations. Whereas many systemic hosts transmitted and encapsidated efficiently, the DI RNAs during BBMV infection of bean or pea plants did not. Similarly, local lesion hosts did not accumulate or encapsidate DI RNAs. The nature of the observed effects on pea and bean hosts was analyzed further by studying the accumulation of DI RNAs in the corresponding mesophyll protoplasts.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Jozef J. Bujarski, Javier Romero

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
general biology - morphology - evolution

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Broad bean mottle virus	Pea (Pisum sativum)
	Broad bean mottle virus	Beans (Phaseolus)