Phytoparasitica (1997) 25, p. 61 (Bol et al.)

J.F. Bol, C.M.A. van Rossum, M.L. Garcia and E.A.G. van der Vossen (1997)
cis-acting coding sequences required for the accumulation of Alfalfa mosaic virus (AlMV) RNAs
Phytoparasitica 25 (1), 61-61
The Xth International Congress of Virology, August 11-16, 1996, Jerusalem, Israel, lecture
Abstract: AlMV RNAs 1 and 2 encode the replicase proteins P1 and P2, respectively. RNA 3 encodes the movement protein P3 and coat protein (CP). CP is expressed from the subgenomic RNA 4. Tobacco has been transformed with cDNA of the P1 gene (P1 plants), P2 gene (P2 plants), or both replicase genes (P12 plants). It was observed that P1 expressed by P1 plants could be used in trans for the replication of RNAs 2 and 3 but not for the replication of RNA 1. Similarly, P2 expressed by P2 plants could be used in trans for replication of RNAs 1 and 3, but not for replication of RNA 2. Studies with P12 protoplasts confirmed that the transgenic P1/P2-replicase accepted inoculum RNA 3 as a template but not RNAs 1 or 2. Mutations in the P3 gene have little effect on RNA 3 accumulation in P12 protoplasts but mutations in the CP gene reduce (+)RNA 3 accumulation 100-fold. The defect in (+)RNA accumulation of a CP mutant could not be complemented in trans by CP of a P3 mutant in doubly infected P12 protoplasts. It is concluded that each of the AlMV RNAs requires an encoded protein in cis, which can be used in trans for the replication of other AlMV RNAs. Apparently, only P3 functions exclusively in trans.
Database assignments for author(s): Maria Laura Garcia

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Alfalfa mosaic virus