Phytoparasitica (1997) 25, p. 235 (Fedorkin et al.)

O.N. Fedorkin, O.N. Denisenko, D.A. Zelenina, S.Yu. Morozov, J.G. Atabekov and A.N. Belozersky (1997)
Evidence for binding of the small putative protein encoded in the area overlapping transport protein and coat protein genes of Tobacco mosaic virus (TMV) with the elongation factor EF-1-alpha
Phytoparasitica 25 (3), 235-235
The Xth International Congress of Virology, August 11-16, 1996, Binyanei haOoma, Jerusalem, Israel, poster
Abstract: We have identified a new gene coding for a putative small, positively charged protein (p4) in TMV (U1, U2 and L strains) genomes. In plant and animal protein-synthesizing systems this protein forms a very stable specific complex (54 kDa) with a protein of 50 kDa. The p4 protein purified from cell-free translational system efficiently binds to the crude preparations of eukaryotic translation factors. Among fractionated translation factors p4 specifically interacts with elongation factor EF-1-alpha. Polyclonal antiserum against p4-DHFR fusion protein and plant factor EF-1-alpha were prepared. Further characterization of the 54 kDa complex was presented and the putative role of p4 in the TMV infection cycle was discussed.
Database assignments for author(s): Sergey Yu. Morozov, Joseph G. Atabekov

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

---

Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Tobacco mosaic virus