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Phytoparasitica (1997) 25, 67-68
F. Revers, T. Candresse, O. Le Gall, S. Souche, H. Lot and J. Dunez (1997)
Molecular and biological variability of lettuce mosaic potyvirus (LMV)
Phytoparasitica 25 (1), 67-68
The Xth International Congress of Virology, August 11-16, 1996, Jerusalem, Israel, poster
Abstract: A collection of ten LMV isolates was characterized by their ability to overcome the three resistance genes described in lettuce cultivars (Lactuca sativa). Several pathotypes could be defined in our study. An immunocapture-RT-PCR assay was developed in conjunction with direct sequencing of the amplified fragments to study the molecular variability of LMV. The N and C terminal regions of the coat protein gene, localized in the 3' part of the genome, and the 5' end region were amplified and sequenced for the ten LMV isolates. Phylogenetic analysis performed using these data showed the existence of two major groups of LMV isolates with limited intra-group variability. Our results indicate that there is little sequence variability among LMV isolates but that biological variability is important. In addition, there is no obvious correlation between molecular and biological variabilities.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Olivier le Gall
Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host
Pest and/or beneficial records:
Beneficial | Pest/Disease/Weed | Crop/Product | Country | Quarant.
|
---|---|---|---|---|
Lettuce mosaic virus | Lettuce (Lactuca) |