Phytoparasitica (1997) 25, 66-67

C.-H. Kim and P. Palukaitis (1997)
The hypersensitive response to Cucumber mosaic virus (CMV) in Vigna unguiculata affects viral RNA accumulation in protoplasts
Phytoparasitica 25 (1), 66-67
The Xth International Congress of Virology, August 11-16, 1996, Jerusalem, Israel, lecture
Abstract: The hypersensitive response (HR) induced by most strains of CMV in cowpea (Vigna unguiculata) was mapped to specific nucleotide changes at positions 1978 and 2007 of Fny-CMV RNA 2. Both nucleotide changes in Fny-CMV RNA 2 to those seen in systemically infecting BCMV, resulted in systemic infection of the mutated Fny-CMV. The double mutant showed increased accumulation of viral RNA in cowpea protoplasts vs Fny-CMV, after 20 h post-inoculation (p.i.), due to a lag in accumulation of Fny-CMV RNAs at 15-18 h p.i. There was also a specific decrease in the Fny-2a protein level after 6 h p.i. Mutation of only Fny-CMV RNA 2 position 1978 yielded a virus that induced an HR that did not inhibit virus spread, leading to systemic necrosis. This mutant showed no lag in viral RNA accumulation in protoplasts. This indicates that there are two separate mechanisms for HR and the inhibition of virus movement.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Peter Palukaitis

Research topic(s) for pests/diseases/weeds:
molecular biology - genes
resistance/tolerance/defence of host

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus