Phytoparasitica (1997) 25, 217-218

I.B. Kaplan and P. Palukaitis (1997)
Characterization of Cucumber mosaic virus (CMV) defective RNA 3 variants generated in tobacco transgenic for the 3a gene
Phytoparasitica 25 (3), 217-218
The Xth International Congress of Virology, August 11-16, 1996, Binyanei haOoma, Jerusalem, Israel, poster
Abstract: Passage of CMV mutants containing small out-of-frame deletions within the 3a gene in tobacco transgenic for the 3a gene, led to the complementation of movement, and the generation of in-frame defective (D) RNA 3 molecules, with various intermediate sized D RNAs observed in earlier passages. The size of the final, stable deletion in the D RNA 3 molecules and the location of the deletion depended on the nature of the initial 3a gene mutation, and appeared to be selected by packaging constraints. Co-infection of the D RNA 3 variants with wildtype CMV led to the loss of the D RNAs in a single passage. In contrast, a naturally arising D RNA 3 could co-exist with the wildtype RNA 3. The individual D RNA 3 variants also showed a hierarchy of selection when co-inoculated with each other. When extracts from individual 3a transgenic plants inoculated with the same frameshift construct were passaged, several classes of different deletion variants were obtained in different plants, suggesting that pooling extracts from several plants may lead to selection of the dominant D RNA 3 variant.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Peter Palukaitis

Research topic(s) for pests/diseases/weeds:
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Cucumber mosaic virus