Pesticide Science (1997) 51, 309-314

Christophe Délye, Frédéric Laigret and Marie-France Corio-Costet (1997)
New tools for studying epidemiology and resistance of grape powdery mildew to DMI fungicides
Pesticide Science 51 (3), 309-314
Abstract: Using a Random Amplified Polymorphic DNA (RAPD) assay, we investigated the genetic polymorphism existing among 62 European isolates of the grape powdery mildew fungus (Uncinula necator [Schw.] Burr.). Isolates overwintering as mycelium in buds were genetically distinct from isolates overwintering as ascospores, suggesting the existence of two genetically isolated powdery mildew populations, and consequently of two independent sources of inoculum in the vineyard. Isolates resistant to fungicides inhibiting sterol 14alpha-demethylation (DMIs) were found in both populations, suggesting that resistance to DMIs may arise independently in the two powdery mildew populations. A PCR assay targeting the gene encoding U. necator 14alpha- demethylase has been developed which will permit an early, specific detection of U. necator infections, and may be useful for spraying programmes.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Database assignments for author(s): Marie-France Corio-Costet, Christophe Delye

Research topic(s) for pests/diseases/weeds:
pesticide resistance of pest
molecular biology - genes

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Erysiphe necator	Grapevine (Vitis)