Pest Management Science (2020) 76, 1626-1638

Adel Khashaveh, Xingkui An, Shuang Shan, Yong Xiao, Qi Wang, Shanning Wang, Zibo Li, Ting Geng, Shaohua Gu and Yongjun Zhang (2020)
Deorphanization of an odorant receptor revealed new bioactive components for green mirid bug Apolygus lucorum (Hemiptera: Miridae)
Pest Management Science 76 (5), 1626-1638
Abstract:
BACKGROUND
The Apolygus lucorum is one of the most destructive insect pests in China with a wide range of host plants. Interaction of A. lucorum with surrounding environment heavily relies on chemical communication. Deorphanization of receptors involved in odors detection elevates our understanding of the olfactory system of this pest and may help to develop a chemical ecology-based control strategy.
RESULTS
AlucOR80, an odorant receptor (OR) in A. lucorum was newly cloned. Gene expression analysis showed that this receptor was mainly expressed in the antennae and head of both sexes but with a male bias. The Xenopus oocytes heterologous expression system coupled with the two-electrode voltage-clamp (TEVC) recording revealed that AlucOR80 was tuned to 21 selected compounds. Furthermore, electroantennogram (EAG) tests confirmed that all 21 ligands of AlucOR80 were electrophysiologically active in antennae of both sexes. Behavioral trials in a three-cage olfactometer indicated that 16 compounds were behaviorally active, amongst which, 12 components were attractants and four components were repellents for adults of both sexes. Butyl butyrate and Dimethyl disulfide (DMDS) were the strongest attractive and repellant compounds, respectively. Importantly, we found the repellency of 1, 8-Cineole, S-(-)-cis-Verbenol and (1S)–(1)-beta-Pinene against adults of A. lucorum.
CONCLUSION
Although AlucOR80 is a general OR, may play important role in the olfactory perception of A. lucorum. Screening of AlucOR80 ligands by behavioral assay provided valuable insights by which olfactory-based management approaches could be developed by utilizing the behaviorally active components as attractants or repellents.
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Link to article at publishers website
Database assignments for author(s): Adel Khashaveh, Qi Wang, Yong-Jun Zhang

Research topic(s) for pests/diseases/weeds:
pheromones/attractants/traps
control - general

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Pest and/or beneficial records:

Beneficial	Pest/Disease/Weed	Crop/Product	Country	Quarant.
	Apolygus lucorum