Pest Management Science (2019) 75, 622-631
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(E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa
Pest Management Science 75 (3), 622-631
Pea aphid (Acyrthosiphon pisum Harris) is one of the major pests in alfalfa crops, causing significant yield losses. (E)-β-farnesene (EβF), an alarm pheromone released by pea aphid, is generic to many species of aphids, and is used to minimize potential danger from predators and parasitoids by avoiding the source of the pheromone.
In this study, EβF synthase gene was constructed into a plant expression vector, and overexpressed in alfalfa (Medicago sativa L.), with expression among transgenic lines confirmed by qRT-PCR. Subcellular localization analysis showed that EβF synthase gene was expressed in the plasma membrane and nucleus of the leaf. GC/MS of extraction from transgenic alfalfa indicated emission of EβF ranging from 5.92 to 13.09 ng day-1 g-1 fresh tissue. Behavior assays in Y-olfactometers demonstrated that transgenic alfalfa expressing AaEβF gene could repel pea aphids, with aphids taking a significantly longer time to select a transgenic line compared with the control line (P < 0.01).
We have demonstrated a potentially valuable strategy of using EβF synthase genes for aphid control in alfalfa.
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Link to article at publishers website
Research topic(s) for pests/diseases/weeds:
control - general
resistance/tolerance/defence of host
Pest and/or beneficial records:
|Acyrthosiphon pisum||Alfalfa/lucerne (Medicago sativa)|