Pest Management Science (2019) 75, 622-631

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Xuemin Wang, Yuhao Gao, Zhihong Chen, Jindong Li, Jianping Huang, Jiangbo Cao, Miaomiao Cui and Liping Ban (2019)
(E)-β-farnesene synthase gene affects aphid behavior in transgenic Medicago sativa
Pest Management Science 75 (3), 622-631
Abstract:
BACKGROUND
Pea aphid (Acyrthosiphon pisum Harris) is one of the major pests in alfalfa crops, causing significant yield losses. (E)-β-farnesene (EβF), an alarm pheromone released by pea aphid, is generic to many species of aphids, and is used to minimize potential danger from predators and parasitoids by avoiding the source of the pheromone.
RESULTS
In this study, EβF synthase gene was constructed into a plant expression vector, and overexpressed in alfalfa (Medicago sativa L.), with expression among transgenic lines confirmed by qRT-PCR. Subcellular localization analysis showed that EβF synthase gene was expressed in the plasma membrane and nucleus of the leaf. GC/MS of extraction from transgenic alfalfa indicated emission of EβF ranging from 5.92 to 13.09 ng day-1 g-1 fresh tissue. Behavior assays in Y-olfactometers demonstrated that transgenic alfalfa expressing AaEβF gene could repel pea aphids, with aphids taking a significantly longer time to select a transgenic line compared with the control line (P < 0.01).
CONCLUSION
We have demonstrated a potentially valuable strategy of using EβF synthase genes for aphid control in alfalfa.
(The abstract is excluded from the Creative Commons licence and has been copied with permission by the publisher.)
Link to article at publishers website


Research topic(s) for pests/diseases/weeds:
control - general
resistance/tolerance/defence of host


Pest and/or beneficial records:

Beneficial Pest/Disease/Weed Crop/Product Country Quarant.
Acyrthosiphon pisum Alfalfa/lucerne (Medicago sativa)